HIGHLIGHTS
- Radical CRC specimens were subjected for histopathological examination. Immunohistochemistry (IHC) was done for the leptin and adiponectin receptors on paraffin embedded tissue sections.
- The tissue IHC expression was analyzed and was correlated with various clinicopathological parameters.
- The results concluded that positive expression of leptin was associated with lymph node spread and advanced stage of CRC.
- Negative expression of adiponectin was associated with metastatic spread of CRC.
ABSTRACT – Background –
Colorectal carcinoma (CRC) is one of the common carcinomas with a rising incidence of metastasis due to its advanced stage of presentation. The existing biomarkers such as CEA (Carcinoembryonic antigen) etc., for prognosis, have low sensitivity and specificity. Hence a need for a newer definitive biomarker. Obesity is the leading cause of CRC. Leptin and adiponectin secreted by adipose tissue have been studied as potential biomarkers in the field of CRC. The present study helps to understand the association of leptin and adiponectin receptors with clinicopathological parameters. Objective – To correlate the various clinicopathological parameters with the tissue expression of leptin and adiponectin receptors in CRC. Methods – It is a cross-sectional prospective study conducted at a tertiary care hospital. Formalin fixed paraffin blocks of all radical resection CRC cases were collected and immunohistochemistry (IHC)was carried out on tumor tissue for leptin and adiponectin receptor. Tumor characteristics and clinical parameters were collected from the hospital medical records. Pearson’s correlation coeff icient test was used. Results – Immunohistochemistry was performed on 60 cases of CRC. Significant positive correlation of leptin was observed with size, lymph node metastasis, advanced stage, and grade of tumor (P<0.05). A significant correlation between adiponectin receptor and CRC was observed concerning age, stage, lymph node metastasis, distant metastasis and grade of tumor. Conclusion – Positive expression of leptin and negative expression of adiponectin receptors in CRC helps to predict the risk of metastasis.
AUTORES
Priyanka PARMESH1, Udupi Shastri DINESH1, Ajay S KHANDAGALE1, Anil Bargale BAPU1, Roshni SADASHIV1 and Pradnya REDDY2
